Responses of soil seed bank and its above-ground vegetation to various reclamation patterns.

Coastal land reclamation has become a primary strategy for alleviating conflicts between human development and land resource utilization. However, anthropogenic activities associated with land reclamation inevitably result in significant changes to coastal wetland ecosystems. Previous studies have mainly focused on the ecological consequences of land reclamation on above-ground vegetation, while overlooking the distinctions between different reclamation patterns and the critical role of soil seed bank in maintaining ecosystem stability. In this study, the responses of soil seed bank and vegetation to various reclamation patterns, as well as the factors influencing changes in seed bank characteristics, were analyzed in a natural coastal wetland (NCW), a reclaimed wetland with sea embankments constructed on native wetland (SEW), and another reclaimed wetland formed through land reclamation from the sea (LRW). These findings suggest that seed banks and their vegetation adopt different adaptation strategies under various reclamation patterns. In the NCW, the proportion of non-halophytes (1.39%), diversity, and density of the seed bank were at their lowest levels, whereas the species compositions derived from the seed bank and vegetation were very similar (similarity coefficient = 0.67). Conversely, the seed bank in the SEW demonstrated the highest species diversity, which differed significantly from the species composition of its above-ground vegetation (similarity coefficient = 0.21). However, the highest proportion of non-halophytes (36.60%), vegetation diversity, and seed bank density occurred in LRW. Furthermore, differences in seed bank characteristics under different reclamation patterns may be related to changes in soil salinity and plant reproductive strategies after reclamation. Adjusting reclamation patterns and restoring soil properties could potentially optimize the types of local plant species and their distribution in reclaimed areas.

Vertical and seasonal dynamics of bacterial pathogenic communities at an aged organic contaminated site: Insights into microbial diversity, composition, interactions, and assembly processes.

Under the background of the Coronavirus Disease 2019 (COVID-19) pandemic, research on pathogens deserves greater attention in the natural environment, especially in the widely distributed contaminated sites with complicated and severe organic pollution. In this study, the community composition and assembly of soil pathogens identified by the newly-developed 16S-based pipeline of multiple bacterial pathogen detection (MBPD) have been investigated on spatiotemporal scales in the selected organic polluted site. We demonstrated that the richness and diversity of the pathogenic communities were primarily controlled by soil depth, while the structure and composition of pathogenic communities varied pronouncedly with seasonal changes, which were driven by the alterations in both physiochemical parameters and organic contaminants over time. Network analysis revealed that the overwhelmingly positive interactions, identified multiple keystone species, and a well-organized modular structure maintained the stability and functionality of the pathogenic communities under environmental pressures. Additionally, the null-model analysis showed that deterministic processes dominated the pathogenic community assembly across soil profiles. In three seasons, stochasticity-dominated processes in spring and summer changed into determinism-dominated processes in winter. These findings extend our knowledge of the response of the bacterial pathogenic community to environmental disruptions brought on by organic contaminated sites.

Significant roles of core prokaryotic microbiota across soil profiles in an organic contaminated site: Insight into microbial assemblage, co-occurrence patterns, and potentially key ecological functions.

Extreme environmental disturbances induced by organic contaminated sites impose serious impacts on soil microbiomes. However, our understanding of the responses of the core microbiota and its ecological roles in organic contaminated sites is limited. In this study, we took a typical organic contaminated site as an example and investigated the composition and structure, assembly mechanisms of core taxa and their roles in key ecological functions across soil profiles. Results presented that core microbiota with a considerably lower number of species (7.93%) than occasional taxa presented comparatively high relative abundances (38.04%) yet, which was mainly comprised of phyla Proteobacteria (49.21%), Actinobacteria (12.36%), Chloroflexi (10.63%), and Firmicutes (8.21%). Furthermore, core microbiota was more influenced by geographical differentiation than environmental filtering, which possessed broader niche widths and stronger phylogenetic signals for ecological preferences than occasional taxa. Null modelling suggested that stochastic processes dominated the assembly of the core taxa and maintained a stable proportion along soil depths. Core microbiota had a greater impact on microbial community stability and possessed higher functional redundancy than occasional taxa. Additionally, the structural equation model illustrated that core taxa played pivotal roles in degrading organic contaminants and maintaining key biogeochemical cycles potentially. Overall, this study deepens our knowledge of the ecology of core microbiota under complicated environmental conditions in organic contaminated sites, and provides a fundamental basis for preserving and potentially utilizing core microbiota to maintain soil health.

The ecological response and distribution characteristics of microorganisms and polycyclic aromatic hydrocarbons in a retired coal gas plant post-thermal remediation site.

Thermal remediation is one of the most common approaches of removing organic pollutants in the retired contamination sites. However, little is known about the performance of bacterial community characteristics after in situ thermal remediation. In this study, the ecological response and spatial distributional characteristics of microorganisms and polycyclic aromatic hydrocarbons (PAHs) were investigated using a high throughput sequencing method in a retired coal gas plant site after in situ thermal remediation in Nanjing, China. Combination of Venn, clustering-correlation heatmap and two - factor correlation network analysis revealed that, microbial communities were obviously affected and classified by soil depths, temperature, and contamination level, respectively. The common and endemic microorganisms of each group were identified. The relative abundances of Thermaerobacter, Calditerricola, Brevibacillus, Ralstonia and Rhodococcus (aerobic bacteria) gradually declined with the increase of soil depth, while those of Bacillus, Fictibacillus, Paenibacillus, Rheinheimera presented opposite tendency. Some thermophilic degradation bacteria of PAHs, including Thermaerobacter, Calditerricola, Bacillus, Rhodococcus, unclassified_p__Firmicutes, Arthrobacter and Deinococcus, were identified and increased in the abundance at heavily polluted sites. Additionally, Proteobacteria, Bacteroidota, Deinococcota, Chloroflexi, Acidobacteriota, and Actinobacteriota showed negative response to the increase of soil depth, temperature and pollution level, while Firmicutes presented a positive response. This implied that Firmicutes has better stress resistance and adaptability to thermal remediation condition. The key environmental factors affecting microorganism composition and distribution were Temperature, Total nitrogen, Oxidation-Reduction Potential, Organic matters, and PAHs concentrations, which explains the dominant driving mechanism of soil depth, temperature, and contamination level on microbial characteristics in thermal remediation site. Our study could contribute to a better understanding of the resilience and adaptation mechanisms of microbial community at the contaminated site after the in situ thermal remediation.

Responses of abundant and rare prokaryotic taxa in a controlled organic contaminated site subjected to vertical pollution-induced disturbances.

Soil microbiota as the key role mediates the natural attenuation process of organic contaminated sites, and therefore illuminating the mechanisms underlying the responses of abundant and rare species is essential for understanding ecological processes, maintaining ecosystem stability, and regulating natural attenuation well. Here, we explored the distributional characteristics, ecological diversities, and co-occurrence patterns of abundant and rare prokaryotic subcommunities using 16S rRNA high-throughput sequencing in vertical soil profiles of a controlled organic contaminated site. Results showed that abundant prokaryotic taxa were widespread across all soil samples, whereas rare counterparts were unbalancedly distributed. Rare subcommunity had more taxonomic groups and higher α- and ß-diversities than abundant subcommunity. Both of these two subcommunities surviving in the organic polluted site possessed the potential of degrading organic contaminants. Abundant subcommunity was little affected by abiotic factors and mainly shaped by soil depth, while rare one was sensitive to environmental disturbances and presented a non-depth-dependent structure. Co-occurrence analysis revealed that rare taxa were more situated at the center of the network and more inclined to cooperate with non-abundant species than abundant taxa, which might play crucial roles in enhancing the resilience and resistance of prokaryotic community and maintaining its structure and stability. Overall, our results suggest that abundant and rare prokaryotic subcommunities present different responses to physicochemical factors and pollution characteristics along vertical soil profiles of organic contaminated sites undergoing natural attenuation.

Pollution pressure and soil depth drive prokaryotic microbial assemblage and co-occurrence patterns in an organic polluted site.

Organic polluted sites have become a global concern of soil contamination, yet little is known about microbial vertical distribution and community assembly in organic polluted sites. Here, high-throughput sequencing technology was employed to investigate prokaryotic microbial diversity and community assembly along soil profile in an abandoned chemical organic contaminated site. Results showed that there was no significant difference (P > 0.05) observed in microbial alpha diversity among different soil layers, whereas the structure of microbial communities presented significantly different (P < 0.05) in the superficial layer (0-0.5 m) compared with intermediate (1-1.5 m) and bottom (2.5-3 m) layers. Soil prokaryotic microbial community evolved to possess the potential of degrading organic pollutants under long-term organic pollution stress. A relatively homogeneous environment created by the organic polluted site mainly induced the ecological process of homogeneous selection driving community assembly, while dispersal limitation gained importance with the increase of soil depth. Organic contaminants were identified as the key driver of destabilizing co-occurrence networks, while the frequent cooperative behaviors among species could combat organic pollution stress and sustain prokaryotic community stability. Collectively, pollution pressure and soil depth jointly affected prokaryotic microbial assemblage and co-occurrence that underpinned the spatial scaling patterns of organic contaminated sites microbiota.

Unraveling the ecological mechanisms of bacterial succession in epiphytic biofilms on Vallisneria natans and Hydrilla verticillata during bioremediation of phenanthrene and pyrene polluted wetland.

In wetland ecosystem, the microbial succession in epiphytic biofilms of submerged macrophytes remains to be fully elucidated, especially submerged macrophytes used to remediate organic pollutants contaminated sediment. Herein, 16 S rRNA gene sequencing was used to investigate the bacterial dynamics and ecological processes in the biofilms of two typical submerged macrophytes (Vallisneria natans and Hydrilla verticillata) settled in sediment polluted by polycyclic aromatic hydrocarbons (PAHs) at two growth periods. The results presented that the variations of bacterial community in the biofilms were influenced by attached surfaces (explanation ratio: 17.30%), incubation time (32.30%) and environmental factors (39.10%). Bacterial community assembly was mainly driven by dispersal limitation which triggered more positive co-occurrence associations in microbial networks, maintaining ecological stability in the process of bioremediation of PAHs. Additionally, the functional redundancy strength of bacterial community was more affected by attached surface than incubation time. The structural equation model illustrated that community assembly drove ß-diversity and explained a part of ecological functions. Environmental factors, community assembly, and ß-diversity jointly affected microbial networks. Overall, our study offers new insights into the microbial ecology in biofilms attached on the submerged macrophytes settled in PAH-polluted sediment, providing important information for deeply understanding submerged macrophyte-biofilm complex and promoting sustainable phytoremediation in shallow lacustrine and marshy ecosystems.

Research trends and hotspots of aquatic biofilms in freshwater environment during the last three decades: a critical review and bibliometric analysis.

Freshwater periphytic biofilms (FPBs), existing widely in various aquatic environments, have attracted extensive attention for many years. In the present study, a bibliometric analysis based on Web of Science Core Collection (WoSCC) was used to understand the research progress, trends, and hot topics of FPBs qualitatively and quantitatively. The results indicated that publications on FPBs have increased from 1991 to 2020 rapidly, and researchers have focused more on the areas of environmental sciences, microbiology, and marine freshwater biology. The most influential countries were mainly the USA, Spain, France, and Germany. Cooperation network analysis reflected that the USA and its affiliated institutions played crucial roles in the research of FPB cooperation, but the collaboration between core author groups still fell short. Based on the analysis of top 20 high-cited FPB documents over the last 30 years, research hotspots mainly included micro-observation and assembly mechanisms of FPBs; interactions of FPBs and pollutants including heavy metals, antibiotic resistance genes, pathogens, organic pollutants, and nanoparticles; and the role of FPBs for biogeochemical cycling, especially nitrogen cycling. Additionally, future research directions were proposed. Overall, this study provides a comprehensive and systematic overview of FPBs, which is useful for research development and researchers who are interested in this area.

Ecological impact of antibiotics on bioremediation performance of constructed wetlands: Microbial and plant dynamics, and potential antibiotic resistance genes hotspots.

Constructed wetlands (CWs) are nature-based solutions for treating domestic and livestock wastewater which may contain residual antibiotics concentration. Antibiotics may exert selection pressure on wetland's microbes, thereby increasing the global antibiotics resistance problems. This review critically examined the chemodynamics of antibiotics and antibiotics resistance genes (ARGs) in CWs. Antibiotics affected the biogeochemical cycling function of microbial communities in CWs and directly disrupted the removal efficiency of total nitrogen, total phosphorus, and chemical oxygen demand by 22%, 9.3%, and 24%, respectively. Since changes in microbial function and structure are linked to the emergence and propagation of antibiotic resistance, antibiotics could adversely affect microbial diversity in CWs. The cyanobacteria community seemed to be particularly vulnerable, while Proteobacteria could resist and persist in antibiotics contaminated wetlands. Antibiotics triggered excitation responses in plants and increased the root activities and exudates. Microbes, plants, and substrates play crucial roles in antibiotic removal. High removal efficiency was exhibited for triclosan (100%) > enrofloxacin (99.8%) > metronidazole (99%) > tetracycline (98.8%) > chlortetracycline (98.4%) > levofloxacin (96.69%) > sulfamethoxazole (91.9%) by the CWs. This review showed that CWs exhibited high antibiotics removal capacity, but the absolute abundance of ARGs increased, suggesting CWs are potential hotspots for ARGs. Future research should focus on specific bacterial response and impact on microbial interactions.

Enhancing remediation of PAH-contaminated soil through coupling electrical resistance heating using Na2S2O8.

Soil polycyclic aromatic hydrocarbons (PAHs) contamination caused by factory relocations is a serious environmental issue across the world. Electrical resistance heating (ERH) and chemical oxidation are two promising in-situ methods for treating volatile and semi-volatile organic pollutants in contaminated soil. Coupling of ERH and chemical oxidation technologies to improve the remediation efficiency for PAH-contaminated soil was estimated in this study. PAH removal ratio in contaminated soils using ERH treatment were significantly negatively correlated with the boiling point of the pollutants (P = 0.002), and 21.63% (DBA high boiling point) to 71.53% (Nap low boiling point) of PAHs in the contaminated soil were removed in 120 min. With oxidant Na2S2O8 coupling, the removal ratio were increased as more oxidant was added. For one Phe, 35.90% was removed by ERH treatment and increased to 52.90% and 79.42% when 0.05 or 2.5 mmol/g oxidant was added, respectively. PAHs with higher boiling points had more obvious removal ratio, such as Bap, which increased from 23.50% to 85.47% when coupling ERH with Na2S2O8, and Phe which increased from 35.90% to 79.42%. Relationships between boiling points and PAH removal ratio changed with coupled oxidants, indicating a change of mechanism from volatilization to coupling effects of volatilization and oxidation with the introduction of Na2S2O8. A dynamic experiment showed that Na2S2O8 can accelerate 45.50% of the treatment process. The results of this research demonstrated a novel, cost-effective coupling approach for remediating soil contaminated by organic pollutants.

The environmental fate of phenanthrene in paddy field system and microbial responses in rhizosphere interface: Effect of water-saving patterns.

The effects of water-saving patterns (Semi-dry water-saving, B; Shallow-wet control irrigation, Q; Traditional flooding irrigation, C; and Moistening irrigation, S) on the environmental fate of phenanthrene (Phe) and microbial responses in rhizosphere were investigated in paddy field system. Results showed the rice grain in Q treatment was more high production and safety with less Phe residue (up to 18%-49%) than other treatments, and the residual Phe in soil declined in the order: C (14.17%) > S (13.36%) > B (5.86%)>Q (2.70%), which proves the existence of optimal water conditions for PAHs degradation and rhizosphere effect during rice cultivation. Laccase (LAC) and dioxygenase (C23O) played important roles in Phe degradation, which were significantly positively correlated with Phe dissipation rate in soil (p < 0.01). Moreover, their activities in Q treatment, rhizosphere and subsoil were higher than those in C treatment, non-rhizoshere and upper layer soil. The introduction of Phe and rice into paddy field system decreased the microorganism diversity, and promoted the activities of enzymes and some PAHs degrading bacteria, such as Delftia, Serratia, Enterobacter, Pseudomonas, norank_f_Rhodospirillaceae, norank_f_Nitrosomonadaceae and so on. According to the cluster analysis, redundancy analysis and correlation analysis between bacterial community composition and environmental factors, water-saving patterns markedly impacted the relative abundance and bacterial community structure by the regulating and controlling on environmental conditions of paddy field. The dioxygenase activity, laccase activity, oxidation-reduction potential and conductivity were the main affecting factors on Phe dissipation during growth stage of rice.

[Adsorption Characteristics of Sulfamethazine on Three Typical Porous High-temperature Modified Solid Waste Materials].

Three typical porous solid wastes, including livestock manure, crop straw, and coal mining waste, were used as raw materials to prepare cattle manure charcoal, straw charcoal, and coal gangue charcoal by low-oxygen controlling temperature carbonization and calcination. Batch adsorption experiments of sulfamethazine (SMZ) in water were carried out. Adsorption kinetics and isothermal adsorption equilibrium were used to investigate the adsorption characteristics of SMZ on cattle dung charcoal, straw charcoal, and coal gangue charcoal, and the adsorption mechanism was discussed by means of field-electron scanning electron microscope, Fourier transform infrared spectroscopy, Boehm titration, Brunauer-Emmett-Teller measurement, and zeta potentiometric titration. The results showed that the adsorption of SMZ on the three carbon materials reached equilibrium at 24 h. The adsorption kinetics of SMZ on three kinds of carbon materials agreed with the quasi-second-order kinetics equation. R2 ranged from 0.9968 to 0.9999, and the adsorption rate decreased with the decrease in effective adsorption sites on the surface of carbon materials. The adsorption process mainly consists of three steps:membrane diffusion, intraparticle diffusion, and the equilibrium stage. Both intraparticle diffusion and membrane diffusion control the adsorption rate. Isothermal adsorption is more consistent with the Freundlich model. R2 is between 0.9874 and 0.9997. It is mainly physical adsorption and spontaneous exothermic reaction. The maximum adsorption capacity of the three kinds of carbon materials was cattle dung carbon (19.64 mg·g-1) > coal gangue carbon (12.06 mg·g-1) > straw carbon (9.16 mg·g-1). The adsorption mechanism of SMZ on the three kinds of carbon materials mainly includes hydrogen bonding between molecules, surface electrostatic adsorption of multi-molecular layers, and pore filling. Of these, electrostatic adsorption is the main adsorption mechanism. The best adsorption performance of cattle manure charcoal may be due to its rich oxygen-containing functional groups, more negative charges, and larger specific surface area and pore volume.

Spatial distribution and assessment of the human health risks of heavy metals in a retired petrochemical industrial area, south China.

Petrochemical industries are widely distributed in China. As a negative consequence, heavy metals in petrochemical area can result in soil contamination. However, the relevant research of heavy metals contamination in petrochemical area was few. In this study, a total of 103 topsoil samples (<20 cm) and 25 profile soil samples were collected and examined in a retired petrochemical industrial area, South China. The results showed the mean contents of Hg, Cd, As, Pb, Ni and Cu were 0.18, 0.69, 16.22, 47.24, 31.62 and 93.06 mg kg-1, respectively. The spatial distribution of six metals in topsoil was largely attributed to the industrial activities during the petroleum refining and transshipment process. Ni was the main pollutant in the petroleum refining process. While, the contamination of other metals mainly were caused by the leakage of the oil during transshipment. The migration of six metals to subsoil layers was also observable. In accordance, Hg, Cd, As, Pb, Cu, and Ni dropped by 95.02, 71.91, 89.45, 90.88, 99.22, and 65.07%, respectively, compared to their contents in topsoil. The contamination of the heavy metals was mainly caused during the process of petroleum refining and transshipment. The distribution of heavy metals in the factory was mainly affected by the industrial activities or the lateral infiltration of Lianhuashan River. Soil ingestion was the primary pathway for children and adults exposure to heavy metals. The total non-cancer human health risk induced by heavy metals was within the limit of USEPA (10-6 a-1). While the cancer risks alone induced by As through soil ingestion to children was 1.14 × 10-6 a-1, which exceeded the limit of USEPA. This study indicated that not only petroleum hydrocarbon but also heavy metals can cause soil contamination in a retired petrochemical industrial area, which provides a novel cognition. Altogether, measures should be taken in practice to substantially improve the soil quality in petrochemical industrial area.

Coupled photocatalytic-bacterial degradation of pyrene: Removal enhancement and bacterial community responses.

Polycyclic aromatic hydrocarbons (PAHs) are a class of pollutants that ubiquitously present in environment and hard to be degraded by microorganisms. Herein, we reported a novel photocatalytic-bacterial coupled removal system to treat PAH-polluted water. Using pyrene as the model pollutant, we demonstrated that the removal percentage of different groups was in order: 63.89% ± 1.03% (Vis-Biological) > 61.27% ± 1.08% (UV-Biological) > 59.58% ± 1.15% (UV) > 57.41% ± 1.13% (Vis) > 6.65% ± 0.72% (Biological) > 1.70% ± 0.34% (Control), showing the coupled system significantly improved the removal percentage of pyrene. Additionally, we observed that the coupled system driven by visible light showed higher removal percentage than UV light, exhibiting a good potential for future application. Sequencing analysis of 16S rRNA genes showed that alpha diversity (richness, evenness and diversity) got promoted and data of the relative abundance showed that Pseudomonadaceae was substituted as the dominant bacteria for Planococcaceae, with some other functional bacteria quickly acclimatizing in the bacterial community. Difference analysis indicated that over half of top fifteen genera were generally different significantly (p < 0.001) among two different samples, and UV light altered structure and composition of bacterial community more than visible light. Functional features' change suggested that the bacterial community not only protected itself but also participated in degrading pyrene. Overall, our study offered a new method for PAH degradation and contributed to further understanding of coupled catalytic-bacterial degradation processes.

Phenanthrene removal and response of bacterial community in the combined system of photocatalysis and PAH-degrading microbial consortium in laboratory system.

This work aimed to study the removal of phenanthrene, change of bacterial community and microbial functions through combined photocatalysis and biodegradation under ultraviolet (UV) and visible light illumination. Results showed that phenanthrene removal was enhanced in combined system irradiated by UV and visible light. High-throughput sequencing of 16S rRNA gene manifested that alpha diversity (richness, evenness and diversity) got promoted and data of relative abundance reported that Planococcaceae as the dominant bacteriawas replaced by Pseudomonadaceae, with some other functional bacteria quickly acclimatizing. Difference analysis indicated that top fifteen genera were generally different significantly (p < 0.001) among two distinct samples, particularly for Pseudomonas on relative abundance. Functional features' regulation suggested that the bacterial community not only protected itself well but also participated in degrading phenanthrene. Selecting suitable degrading microbial consortium from natural environment and understanding deeply on performance of bacterial community contribute to assemble effective and directional combined system.

Cu/N-codoped TiO2 prepared by the sol-gel method for phenanthrene removal under visible light irradiation.

Cu/N-codoped TiO2 nanoparticles were prepared by the modified sol-gel method, to study its efficiency for the removing of polyaromatic hydrocarbon (phenanthrene) from an aqueous solution. Urea and copper sulfate pentahydrate were used as sources of doping element for Cu/N-codoped TiO2, respectively. The characterizations of the nanoparticles were done by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and UV-vis diffuse reflectance spectra. XRD revealed that all the nanoparticles were indexed to the anatase phase structure, with crystallite size range from 11 to 30 nm, which decreased with the doping of copper and nitrogen. The photocatalytic activities of Cu/N-codoped TiO2 showed the highest activities than other TiO2 nanoparticles (TiO2 and N-doped TiO2). The photodegradation efficiency of Cu/N-codoped TiO2 on phenanthrene under visible light irradiation was slightly higher (96%) comparing to UV light irradiation (94%). Cu/N-codoped TiO2 was found to be very efficient and economical for phenanthrene removal, because the smallest amount of Cu/N-codoped TiO2 exhibited the best removal efficiency on phenanthrene.

Correction to: Cu/N-codoped TiO2 prepared by the sol-gel method for phenanthrene removal under visible light irradiation.

The article "Cu/N-codoped TiO2 prepared by the sol-gel method for phenanthrene removal under visible light irradiation," written by Zhenhua Zhao, Abduelrahman Adam Omer, Zhirui Qin, Salaheldein Osman, Liling Xia, and Rajendra Prasad Singh.

The dissipation and risk alleviation mechanism of PAHs and nitrogen in constructed wetlands: The role of submerged macrophytes and their biofilms-leaves.

The role of submerged macrophytes (Vallisneria natans, Hydrilla verticillata and artificial plant) and their biofilms-leaves for the dissipation and risk alleviation mechanism of PAHs (phenanthrene and pyrene) and nitrogen in constructed wetland systems with PAH-polluted sediments were investigated. Biofilms-leaves/surface might contribute to PAHs degradation, which was positively correlated with PAHs degrading bacteria. Nitrogen-fixing bacteria in biofilms on surface might cause total nitrogen in sediment (TNs) increasing by 4% from 14th d to 28th d indirectly when suffering PAHs pollution. The relative abundance of nitrogen-fixing bacteria significantly increased with the increase of PAHs concentrations in early period (p < 0.01), which might lead to risk of nitrogen accumulation further. Heat maps showed that the relative abundance of functional bacteria were influenced in order of attached surface > incubation time > spiking concentration of PAHs. Interestingly, differences of deduced bacterial functions were affected in order of incubation time > attached surface > spiking concentration. Thus, submerged macrophytes and their biofilms on leaves not only played an important role in PAHs degradation, but also regulated the nitrogen cycling in constructed wetland systems, which could reduce these pollutants risk for natural environment, organisms and human health.

The responding and ecological contribution of biofilm-leaves of submerged macrophytes on phenanthrene dissipation in sediments.

The bacterial communities and ecological contribution of biofilm-leaves of the Vallisneria natans (VN), Hydrilla verticillata (HV) and artificial plant (AP) settled in sediments with different polluted levels of phenanthrene were investigated by high-throughput sequencing in different growth periods. There was no significant difference among the detected Alpha diversity indices based on three classification, attached surface, spiking concentration and incubation time. While Beta diversity analysis assessed by PCoA on operational taxonomic units (OTU) indicated that bacterial community structures were significantly influenced in order of attached surface > incubation time > spiking concentration of phenanthrene in sediment. Moreover, the results of hierarchical dendrograms and heat maps at genus level were consistent with PCoA analysis. We speculated that the weak influence of phenanthrene spiking concentration in sediment might be related to lower concentration and smaller concentration gradient of phenanthrene in leaves. Meanwhile, difference analysis suggested that attached surface was inclined to influence the rare genera up to significant level than incubation time. In general, the results proved that phenanthrene concentrations, submerged macrophytes categories and incubation time did influence the bacterial community of biofilm-leaves. In turn, results also showed a non-negligible ecological contribution of biofilm-leaves in dissipating the phenanthrene in sediments (>13.2%-17.1%) in contrast with rhizosphere remediation (2.5%-3.2% for HV and 9.9%-10.6% for VN).

Dissipation characteristics of pyrene and ecological contribution of submerged macrophytes and their biofilms-leaves in constructed wetland.

The dissipation characteristics of pyrene and ecological contribution of submerged macrophytes (Vallisneria natans, Hydrilla verticillata and artificial plant) and their biofilms-leaves in constructed wetland system were investigated by high-throughput sequencing. Results showed a non-negligible ecological contribution of submerged macrophytes and their biofilms-leaves in dissipating pyrene in planted sediments (33.25-43.00%) in contrast with unplanted system (23.25-26.50%). Alpha and Beta diversity analysis indicated that bacterial community structures were influenced in order of attached surface > incubation time > spiking concentration of pyrene in sediment. Difference analysis suggested that attached surface tended to affect the rare genera up to significant level compared with incubation time. In addition, RDA analysis showed that PAH-degrading bacteria in biofilms-leaves of submerged macrophytes were positively correlated with the dissipation ratios of pyrene. Interestingly, nitrogen transforming microorganisms were also influenced in the presence of pyrene, while submerged macrophytes contributed to alleviate the impact.